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Assertion : Gel electrophoresis and elut...

Assertion : Gel electrophoresis and elution are two important processes.
Reason : After staining with ethidium bromide it has to be exposed to U.V light.

A

If both the assertion and the reason are true and the reason is a correct explanation of the assertion

B

If both the assertion and reason are true but the reason is not a correct explanation of the assertion

C

If the assertion is true but the reason is false

D

If both the assertion and reason are false

Text Solution

Verified by Experts

The technique which separate DNA fragments based on their size is called gel electrophoresis. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. The DNA fragments can be seen as bright orange coloured bands. These separated bands are cut out from agarose gel and extracted from the gel piece, this is called elution. The purified DNA fragments can be used in constructing recombinant DNA by joining them with cloning vectors.
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