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Hardening in tissue culture is...

Hardening in tissue culture is

A

Keeping at 30-50°C temperature for about 30 minutes

B

Acclimatisation of tissue culture plants slowly before growing in the field

C

Plunging the vials into water at 37–40°C

D

None of the above

Text Solution

Verified by Experts

The correct Answer is:
B
Hardening involves gradual acclimatization of invitro grown plants to invivo condition. The plantlets are immediately irrigated with nutrient solution and kept in hardeny room where light, humidity and temperature are maintained
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