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Sterilization of tissue culture medium i...

Sterilization of tissue culture medium is done by

A

Autoclaving of medium at 120°C for 15 minutes

B

Filtering the medium through fine sieve

C

Mixing the medium with antifungal agents

D

Keeping the medium at – 20°C

Text Solution

Verified by Experts

The correct Answer is:
A

Sterilization to make culture free from microbes needs to be treated with specific anti-microbial chemicals & the vessels, media and instruments are suitably treated with steam (autoclave) at 120°C for 15 minutes
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