DNA Replication|Meselson And Stahl's Experiment|OMR|Enzymes

DNA Replication|Meselson And Stahl's Experiment|Enzymes |OMR

DNA Replication Introduction Semi-Conservative Mode OF DNA Replication Messelson And Stahls Experiment Questions Based On DNA Replication (Semiconservative Mode)

Properties Of Genetic Material|How DNA Is Replicated?|Messelson And Stahl's Experiment|Questions|OMR

DNA Replication|Enzymes|OMR

Draw a neat and labelled diagram explaining Meselson’s and Stahl’s experiment.

Discuss the singnificance of heavy isotope of nitrogen in the Meselson and Stahl's experiment .

State the aim and describe Messelson and Stahl's experiment.

The semi-conservative nature of DNA replication was established by Meselson and Stahl in their classic experiment with bacteria. They grew bacteria in N^(15)-NH_(4)CI containing medium with N_(14) - containing compounds and allowed to grow for three generation . CsCI density gradient centrifugation of isolated DNA established the nature of semiconservation DNA replication. The pictorial representation below shows the positon of differentially labeled DNA in CsCI density gradient. Had the DNA replication been conservative, what would have been the pattern ?

Answer the following questions based on Meselson and Stahl's experiment of E. coli : (a) Write the name of the chemical substance used as the only source of nitrogen in the experiment. (b) Why did they allow the synthesis of the light and the heavy DNA molecules in the organism ? (c ) How did they distinguish the heavy DNA molecules from the light DNA molecules? Explain. (d) Write the conclusion the scientists arrived at, at the end of the experiment.

Describe Meselson and Stahl's experiment to show that DNA replication is semiconsertive.