The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

Options

A. Non-recombinant bacteria containing betagalactosidase
B. Insertional inactivation of alphagalactosidase in non-recombinant bacteria
C. Insertional inactivation of alphagalactosidase in recombinant bacteria
D. Inactivation of glycosidase enzyme in recombinant bacteria

Answer

A

Explanation

Lac operon Z-gene produce beta galactosidase that convert X-gal chromogen into blue colour in non recombinant bacteria if this gene is affected by insertion inactivation this reaction does not take place and bacteria appears white. But this inactivation take place in beta-galactosidase gene not in `alpha`-galactosidase gene in recombinant bacteria so right answer is non recombinant bacteria containing beta galactosidase enzyme that cause blue colour colonies of bacteria.