The following is generally used for crea...

The following is generally used for creating density gradient during centrifugation

NaCl

KCl

CsCl

`MgCl_(2)`

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Repeitive DNA are separated fom bulk geneomic DNA as different peak during density gradient centrifugation. The bulk DNA forms a major peaks and the other small peaks are refferred to as

The semi-conservative nature of DNA replication was established by Meselson and Stahl in their classic experiment with bacteria. They grew bacteria in N^(15)-NH_(4)CI containing medium with N_(14) - containing compounds and allowed to grow for three generation . CsCI density gradient centrifugation of isolated DNA established the nature of semiconservation DNA replication. The pictorial representation below shows the positon of differentially labeled DNA in CsCI density gradient. Had the DNA replication been conservative, what would have been the pattern ?

Replication was allowed to take place in the presence of redioactive deoxynucleotides precursors in E. coli that was a mutant for DNA ligase. Nswly synthesised redioactive DNA was purified and strands were separated by denatureation. These were centrifuged using density gradient centrifugation. Which of the following would be a correct resulat ?

Assertion :- The repetitive DNA are separted from bulk genomic DNA as different peaks during density geadient centrifugation. Reason : Type bulk DNA forms a minor peak %+& other major peaks are referred to as satellite DNA.

Assertion :- Tylor and colleages in 1958 used CsCl density gradient centrifugation technique to prove semiconservatie DNA replication. Reason : They use ""^(15)N heavy nitrogen which is not a radioactive isotope.

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