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Type-I restriction enzyme...

Type-I restriction enzyme

A

recognise the specific seqences and cleave DNA at the specific

B

do not recognise or cleave the specific sequence

C

recognise specific sequence but cleave non-specific sequence

D

None of the above

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The correct Answer is:
b
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Type-II restriction enzymes are preferred over type-I in cleaving DNA strand because they

Restriction enzymes

Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endemicleases are Type 1. Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A. Only type II restriction enzymes are used in gene manipulation because:

The restriction enzyme Hind II was isolated from bacterium

Restriction Enzymes

Restriction enzymes are :

ARIHANT NEET-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-Chapter exercises (Taking it together (Assorted Questions of the Chapter for advanced Level Practice ))
  1. In the embryo of cloning Dolly sheep, the fusion is done between

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  2. Molecular cloning is

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  3. Type-I restriction enzyme

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  4. Type-II restriction enzymes are preferred over type-I in cleaving DNA ...

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  5. Restriction enzymes are used in genetic engineering because they

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  6. A fragment of DNA, cut by a restriction enzyme, forms bonds with other...

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  7. It is preferable to use yeast rather than bacteria as recipient cells ...

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  8. Which of the followings is first recombinant DNA produced unintentionl...

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  9. The role of restriction enzymes in DNA technology is to

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  10. Gene for cloning may be chemically sunthesised

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  11. Yeast has become important in genetic engineering because it.

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  12. You are attempting to introduce a gene that imports larval moth resist...

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  13. Complementary DNA does not create a momplete gene library because

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  14. Choose the correct option regarding retrovirus

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  15. Choose the incorrect match.

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  16. Using recombiant DNA technology, genes from a donor cell can be insert...

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  17. In recombinant DNA technology, a plasmid vector is cleaved by

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  18. Which of the following is not true for recognition sequences?

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  19. Significance of 'heat shock' method in bacterial transformation is to ...

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  20. Which of the following steps are catalysed by Taq polymerase in a PCR ...

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