A fragment of DNA, cut by a restriction ...

A fragment of DNA, cut by a restriction enzyme, forms bonds with other DNA molecules that have

methyl groups attached to them

unpaired bases

plasmid components

been fragmented by the same restrction enzyme

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DNA fragments cut by restriction enzyme can be separated by

The enzyme used to cut the DNA molecule is :

The restriction endonuclease enzyme binds to the DNA and cuts

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

The role of restriction enzymes in DNA technology is to

Restriction enzyme EcoR I cleavages DNA at the sequence

How many fragments will be generated if you digest a linear DNA molecule with a restriction enzyme having four recongnition sites on the DNA?

When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky-ends and these can be joined together (end to end) using

Assertion: The recombinant DNA molecule cannot be created, unless one cuts the vector & source DNA with same restriction enzyme Reason :When cuts by same enzyme both will have same sticky ends, that can be joined by DNA ligase

ARIHANT NEET-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-Chapter exercises (Taking it together (Assorted Questions of the Chapter for advanced Level Practice ))

Type-II restriction enzymes are preferred over type-I in cleaving DNA ...
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