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Assertion : Gel electrophoresis and elut...

Assertion : Gel electrophoresis and elution are two important processes.
Reason : After staining with ethidium bromide it has to be exposed to U.V light.

A

Only A is correct

B

Both A and B are correct

C

Only B is correct

D

Only A is correct and B is incorrect

Text Solution

Verified by Experts

The correct Answer is:
b
Agarose gel electrophoresis plays a critical role in analysing DNA in laboratory experiments. It is a method of separating biological moecules using an electrical current.
Ethidium bromide is a molecule commonly used to visualise DNA in agarose gel electrophoresis experiments. It both binds to DNA and fluorescence under the proper conditions. Ethidium bromide is known as an intercalating agent.
Elution is separation of material by washing and mixing them with water or bufer solution, in order to separate the heavier costituents, which settle out in solution, from the either.
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