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Some foreign DNA fragment is attached to...

Some foreign DNA fragment is attached to Cla I site of pB322. This recombinant vector is used to transit Escherichia coli host cells. The cell subjected to transformation are plated on two different media-one containing ampicillin and the other containing tetracycline. THe transformed cells containing the recombinant vector

A

will grow on both tetracycline containing and ampicillin containing media

B

will not grow on either tetracycline containing or ampicillin containing media

C

will grow on tertracycline, but not on ampicillin containing medium.

D

will grow on ampicillin, but not on tetracycline containing medium

Text Solution

Verified by Experts

The correct Answer is:
d
The transformed cell will grow on ampicillin but not on tetracycline. This is because the foreign DNA fragment is attached to Cla I site, which is present in the region of `tet^(R )` gene. Thus, it cause disintegration of `tet^(R )` gene.
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