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A mixture containing DNA fragments a, b,...

A mixture containing DNA fragments a, b, c and d with molecular weigths of `a+b=c,a gt b` and `d gt c`, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anode sides of the gel would be

A

b, a, c, d

B

a, b, c, d

C

c, b, a, d

D

b, a, d, c

Text Solution

Verified by Experts

The correct Answer is:
a

The positions of these DNA fragments on the electrophoresis gel depends upon relative molecular weight. The fragment with lowest molecular weight will appear farther from the wall and fragment with higher molecular weight will appear near the wall. So, on the basis of information given, molecular weight of d is maximum and molecular weight of b is minimum. SO, the order of fragments will be (b, a, c, d).
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