Insect pest resistant Bt-cotton plant was developed using:

Options

A. somaclonal variation
B. micropropagation
C. somatic hybridisation
D. transgenic technology

Answer

D

Explanation

Bt cotton is a genetically modified variety of cotton producing an insecticide. It was produced by Monsanto by inserting cry gene coding for Bt toxin into cotton plant gemone by using transgenic technology causing cotton to produce this natural insecticide in its tissues.
This gene was obtained from Bacillus thuringiensis, while other techniques mentioned in the options such as somaclonal variation, somatic hybridisation and micropropagation are the methods that rely on tissue culture techniques of producing plants by using different plant parts. They do not involve the gene transfer.