Summarize the process by which the sequence of DNA bases in Human Genome Project was determined using the method developed by Frederick Sanger. Name a free living non-pathogenic nematode whose DNA has been completely sequenced.

Which of the following is true about the method of sequencing DNA fragments developed by Frederick Sanger?

The main cloning vectors used for DNA sequencing in the Human Genome Project are _________.

The main cloning vectors used for DNA sequencing in the Human Genome Project are _________.

Assertion. Human Genome Project was a mega project launched to find out the complete DNA sequence of human genome. Reason. It was possible only with the help of genetic engineering techniques to isolate and clone any piece of DNA and fast techniques for determining DNA sequences.

The quest for finding out the complete DNA sequence of human genome lead to a mega project called HGP. Expand HGP.

Match each term on the right with the definition on the left, Column - I A) Bacterial enzymes used to cut DNA at defined sequences B) Sequences cut by restriction enzymes C) Ends left on DNA segments cut by DNA restriction enzymes D) Circular pieces of DNA found in bacteria E) Bacterial viruses F) Process by which bacteria take up pieces of DNA from the environment G) Plasmid DNA that has incorporated human DNA H) Term used to describe any vehicle that moves DNA from the organism to another Column - II I) Transformation II) Cloning vector III) Recombinant IV) Recognition sequences V) Plasmids VI) Sticky ends VII) Restriction enzymes VIII) Bacteriophages

Match each term column-I with the definition in column-II, Column - I A) Bacterial enzymes used to cut DNA at defined sequences B) Sequences cut by restriction enzymes C) Ends left on DNA segments cut by DNA restriction enzymes D) Circular pieces of DNA found in bacteria E) Bacterial viruses F) Process by which bacteria take up pieces of DNA from the environment G) Plasmid DNA that has incorporated human DNA H) Term used to describe any vehicle that moves DNA from the organism to another Column - II I) Transformation II) Cloning vector III) Recombinant IV) Recognition sequences V) Plasmids VI) Sticky ends VII) Restriction enzymes VIII) Bacteriophages