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Deoxyribonucleotides....

Deoxyribonucleotides.

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(1)A nucleotide is the structural unit of the nucleic acid.Deoxyribonucleotides are units in the DNA molecule.(2)Each Deoxyribonucleotide has three components,viz,pentose sugar called deoxyribose,phosphate group and nitrogen base.(3)Deoxyribose sugar(`C_(5)H_(10)O_(5)`) is a five carbon compound having pentagonal ring structure.(4)Phosphate group is phosphate acid(`H_(3)PO_(4)`) having three active-OH groups.Two of these are involved in the strand formation,(5)nitrogen bases are of two main types,viz.,purines and pyrimidines,viz.,adenine(A)and guanine(G).Pyrimidines are single ring compounds and are of two types,viz.,cytosine(C) and thymine(T).(6)Nucleotides are named according to the nitrogenous base that they contain .E.g thymine nucleotide,guanine nucleotide,cytosine nucleotide,adenine nucleotide,etc(7)In a nucleotide,there are glycosidic bonds between sugar and nitogen base and phosphodiester bond between phosphate and sugar molecule.(8)Sugar and nitrogen base together are called nucleoside whereas phosphate added to nucleoside froms a nucleotide.
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A schematic representation of polymerase chain reaction (PCR) up to the extension stage is given below. Which of the following has popularized the PCR (Polymerase Chain Reaction) ? a) Easy availability of DNA template b) Availability of synthetic primers c) Availability of cheap deoxyribonucleotides d) Availability of thermostable DNA polymerase.

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