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A biotechnologist wanted to create a col...

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA ?

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Which one of the following restriction enzyme can cut "Tet"^R site of pBR322 ?

Which one of the following restriction enzyme can cut "Tet"^R site of pBR322 ?

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explain pBR 322 plasmid and label the following in it: Any two restriction sites.

In pBR322, tetracycline resistance gene (tet^(R)) has recognition site for which of the following restriction endonuclease ?

In order to insert alien DNA at the site of tetracycline resistance gene in pBR322 the restriction endonuclease used can be :

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