What is the first step in the Southern Blot technique

Options

A. Denaturation of DNA on the gel for hybridization with specific prode
B. Production of a group of genetically idenatial cells
C. Digestion of DNA by restriction enzyme
D. Isolation of DNA from a nucleated cell such as the one from the scene of crime

Answer

d

Explanation

d) The first step in southern blot technique is isolation of DNA from a nucleated cell and then these isolated DNA sequences are transferred to a nylon or nitrocellulose sheet placed over the gel.