Which one of the following technique is not used for early molecular diagnosis ?

Options

A. Polymerase chain reaction
B. Recombinant DNA technology
C. Polyacrylamide gel electrophoresis
D. Enzyme linked immunosorbent assay

Answer

To determine which technique is not used for early molecular diagnosis, we can analyze each of the provided options step by step.

### Step-by-Step Solution:

1. **Understand the Question**: We need to identify which technique among the given options is not utilized for early molecular diagnosis.

2. **Analyze Option A: Polymerase Chain Reaction (PCR)**:
- PCR is a widely used technique in molecular biology that allows for the amplification of DNA.
- It is commonly used in early molecular diagnosis for detecting pathogens by amplifying their genetic material.
- **Conclusion**: PCR is used for early molecular diagnosis.

3. **Analyze Option B: Recombinant DNA Technology**:
- Recombinant DNA technology involves combining DNA from different organisms to create new genetic combinations.
- This technique is primarily used in genetic engineering rather than for diagnostic purposes.
- **Conclusion**: Recombinant DNA technology is not used for early molecular diagnosis.

4. **Analyze Option C: Polyacrylamide Gel Electrophoresis (PAGE)**:
- PAGE is a technique used to separate macromolecules like proteins and nucleic acids based on size and charge.
- It is often used in conjunction with other techniques for analyzing RNA and DNA samples.
- **Conclusion**: PAGE can be used in molecular diagnosis.

5. **Analyze Option D: Enzyme-Linked Immunosorbent Assay (ELISA)**:
- ELISA is a biochemical technique used to detect the presence of antibodies or antigens in a sample.
- It is commonly employed in diagnosing diseases such as HIV.
- **Conclusion**: ELISA is used for early molecular diagnosis.

### Final Conclusion:
Based on the analysis, the technique that is not used for early molecular diagnosis is **Recombinant DNA Technology (Option B)**.