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Restriction endonucleases...

Restriction endonucleases

A

tissue culture

B

genetic engineering

C

cell fractionation

D

regeneration of tissue

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The correct Answer is:
B
There are three general types of restriction enzyme . Type I and III cleave DNA away from recognition sites . Types II restriction endonucleases cleave DNA at specific recognition sites.
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Restriction endonuclease are utilized in genetic engineering as :

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Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endemicleases are Type 1. Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A. Which type of restriction endonucleases is used most in genetic engineering?

GRB PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-Exemplar Problems
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