Plasmid :

A

c-DNA

B

viral RNA

C

mitochondrial DNA

D

cirular extrachromosomal DNA in bacteria

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The correct Answer is:
D
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Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium. (i) Transform bacteria with recombinant (ii) Cut the plasmid DNA using restriction enzyme . (iii) Extract plasmid DNA to - DNA fragments DNA (iv) Bind the plasmid DNA to non-plasmid DNA fragments . (v) Use ligase to seal plasmid DNA to non- plasmid DNA Arrange the above steps in the right sequence and select the CORRECT option

I. Transform bacteria with recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacteria cells. IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligate to seal plasmid DNA to non-plasmid DNA. From the given list, which of the following is the most logical sequence of steps for splicing foregain DNA into a plasmid and inserting the plasmid into a bacterium ?

GRB PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-Exemplar Problems
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  6. Restriction' in restriction enzyme refers to

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  8. In agarose gel eletrophoresis DNA molecules are separated on the basis...

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  9. The most important feature in a plasmid to be used as a vector is :

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  11. Which of the following has popularised the PCR (polymerase chain React...

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  13. Significance of 'heat shock' method in bacterial transformation is to ...

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  14. The role of DNA ligase in the construction of a recombinant DNA molec...

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  15. Which of the following bacteria is not a source of restriction endonu...

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  16. Which of the following steps are catalysed by Taq polymerase in PCR ...

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  17. A bacterial cell was transformed with a recombinant DNA that was gener...

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  18. Which of the following should be chosen for best yield if one were to ...

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  19. Who among the following was awarded the Nobal Prize for the developmen...

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  20. Which of the following statements does not hold true for restriction e...

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