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Some of the step involved in the product...

Some of the step involved in the production of humulin are given below. Arrange them in the correct sequence and select the correct option
(i) Synthesis of gene (DNA) for human insulin artifically
(ii) Culturing recombinant E.coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into plasmid
(vi) Etraction of recombinant gene product from E.coli.

A

ii, `i`, iv, iii, v, vi

B

`i`, iii, v, vi, ii, iv

C

`i`, iv, v, ii, vi, iii

D

iii, v, ii, `i`, vi, iv

Text Solution

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The correct Answer is:
C
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Some of the steps involved in production of humulin are given below. Choose the correct sequence (i) Synthesis of insulin gene artificially (ii) Culturing recombinant E. coli in bioreactors (iii) Purification of humulin (iv) Insertion and human insulin gene into plasmid (v) Introduction of recombinant plasmid into E. coli (vi) Extraction of recombinant gene product from E. coli.

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