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What are the enzymes involved in DNA rep...

What are the enzymes involved in DNA replication in eukaryotes?

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(i) The enzyme helicases are involved in unwinding of DNA by breaking hydrogen bonds holding the two strands of DNA.
(ii) Topoisomerase is an enzyme which breaks DNAs covalent bonds and removes positive supercoiling ahead of replication fork.
(iii) DNA replication is initiated by an enzyme DNA polymerase `alpha` / primase which synthesizes short stretch of RNA primers on both leading strand (continuous DNA strand) and laggirtg strands (discontinuous DNA strand).
(iv) DNA Pol `alpha` (alpha), DNA Pol `delta` (delta) and DNA Pol `epsilon` (Epsilon) are the 3 enzymes involved in nuclear DNA replication.
(v) DNA Pol `alpha` - Synthesizes short primers of RNA
(vi) DNA Pol `delta` - Main Replicating enzyme of cell nucleus
(vii) DNA Pol `epsilon` - Extend the DNA Strands in replication fork
(viii) DNA ligase joins any nicks in the DNA by forming a phosphodiester bond between 3' hydroxyl and 5' phosphate group.
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