Isolation of the genetic material in pure form, free from other macromolecules can be achieved by treating the bacterial cells/plant or animal tissues with the following enzymes, except

Options

A. lysozyme
B. cellulase
C. chitinase
D. ligase

Answer

To solve the question regarding the isolation of genetic material in pure form from bacterial cells, plant, or animal tissues, we need to identify which enzyme is not used for this purpose.

### Step-by-Step Solution:

1. **Understand the Question**: We need to isolate genetic material (DNA) from various sources (bacterial cells, plant cells, or animal tissues) and identify the enzyme that is NOT used for this isolation.

2. **Review the Enzymes**:
- **Lysozyme**: This enzyme is known to break down the peptidoglycan layer of bacterial cell walls. It is effective in lysing bacterial cells, making it useful for isolating DNA from bacteria.
- **Cellulase**: This enzyme digests cellulose, which is the main component of plant cell walls. It is used to break down plant tissues to isolate DNA from plant cells.
- **Chitinase**: This enzyme is used to break down chitin, which is found in the cell walls of fungi. It is useful for isolating DNA from fungal cells.
- **Ligase**: This enzyme is responsible for joining DNA fragments together. It is not used for the isolation of DNA, but rather for processes that involve manipulating DNA after it has been isolated.

3. **Identify the Exception**: Among the enzymes listed, ligase is the only enzyme that does not aid in the isolation of genetic material. Instead, it is used in DNA manipulation processes.

4. **Conclusion**: The enzyme that is NOT used for isolating genetic material in pure form is **ligase**.

### Final Answer:
The enzyme that is NOT used for isolating genetic material in pure form is **ligase**.

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