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In cloning vector pBR322, the Barn HI cl...

In cloning vector pBR322, the Barn HI cloning site is found in which selectable marker gene?

A

Ampicillin resistance gene

B

Tetracycline resistance gene

C

Chloramphenicol resistance gene

D

Lac Z gene

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The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding Cloning Vectors**: Cloning vectors are DNA molecules used to transport foreign genetic material into a host cell. They typically contain essential features such as an origin of replication (ORI) and selectable marker genes. 2. **Identifying Selectable Marker Genes**: In the context of the cloning vector pBR322, there are two main selectable marker genes: the ampicillin-resistant gene (amp^R) and the tetracycline-resistant gene (tet^R). These genes allow for the selection of cells that have taken up the vector by providing resistance to specific antibiotics. 3. **Recognizing the BamHI Cloning Site**: The BamHI restriction enzyme is used to create a cloning site within the vector. This site is where foreign DNA can be inserted. 4. **Locating the BamHI Site in pBR322**: In pBR322, the BamHI cloning site is specifically located within the tetracycline-resistant gene (tet^R). This means that when foreign DNA is inserted at this site, it disrupts the tetracycline resistance, allowing for the selection of successful recombinants. 5. **Conclusion**: Therefore, the BamHI cloning site in the cloning vector pBR322 is found in the tetracycline-resistant gene. **Final Answer**: The BamHI cloning site is found in the tetracycline-resistant gene. ---
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