Write the brief note on chromatography.

Explanation

Chromatography is a method of separation of compontents of a mixture between the stationary phase and a mobile phase.
Chromatography involves the following three steps :
1) Absorption and retention of a mixture of substances on the stationary phase and separation of adsorbed substances by the mobile phase to different distance on the stationary phase.
2) Recovery of hte substances separated by a continuous flow of the mobile phase (known as elution) and
3) Qualitative and quantitative analysis of the eluted substances.
Based on the physical states of the stationary phase and mobile phase and also on the basic of the principle of adsorption of substances on the stationary phase chromatography processes are classified into several types as given below.

There are two chromatography techniques
(1) Adsorption chromatography (2) Partition chromatography.
There are again two types of Adsorption chromatography techniques. They are (1) Column chromatography (2) Thin layer chromatography.