Which one is the correct sequene of molecules during biological nitrogen fixation?

Options

A. Nitrogen - diamide - hydrazine - ammonia
B. Nitrogen - hydrazine - dimide - ammonia
C. Nitrogen - ammonia - a ketoglutraic acid - glutamic acid
D. Nitrogen - nitrite - nitrate - amino acid

Answer

### Step-by-Step Solution

1. **Understanding Biological Nitrogen Fixation**:
- Biological nitrogen fixation is the process by which atmospheric nitrogen (N₂) is converted into ammonia (NH₃) by certain microorganisms, primarily prokaryotes, using the enzyme nitrogenase.

2. **Identifying the Molecules Involved**:
- The key molecules involved in this process include:
- Nitrogen (N₂)
- Hydrazine (N₂H₄)
- Diamide (NH₂CONH₂)
- Ammonia (NH₃)

3. **Determining the Sequence**:
- The correct sequence of molecules during biological nitrogen fixation is:
- **Nitrogen (N₂)** → **Hydrazine (N₂H₄)** → **Diamide (NH₂CONH₂)** → **Ammonia (NH₃)**

4. **Evaluating the Options**:
- The options provided were:
1. Nitrogen, Diamide, Hydrazine, Ammonia
2. Nitrogen, Hydrazine, Diamide, Ammonia
3. Nitrogen, Ammonia, Alpha Keto, Glutaric Acid, Glutamic Acid
4. Nitrogen, Nitrite, Nitrate, and Amino Acid
- Based on the correct sequence identified, the second option "Nitrogen, Hydrazine, Diamide, Ammonia" is the correct answer.

5. **Conclusion**:
- The correct sequence of molecules during biological nitrogen fixation is **Nitrogen → Hydrazine → Diamide → Ammonia**.