Virus free plants from a virus infected plant can be obtained through:

Options

A. Root tip culture
B. Shoot tip culture
C. Callus culture
D. Suspension culture

Answer

**Step-by-Step Solution:**

1. **Understanding the Problem**: The question asks how to obtain virus-free plants from a virus-infected plant. This involves identifying a method that can isolate healthy plant tissue that is free from the virus.

2. **Identifying Tissue Culture Methods**: The methods mentioned include root tip culture, shoot tip culture, callus culture, and suspension culture. We need to determine which of these methods can effectively yield virus-free plants.

3. **Focusing on Meristematic Tissue**: The key to obtaining virus-free plants lies in the meristematic tissue, which is located at the shoot and root tips. This tissue is known to be free from viruses because it consists of actively dividing cells that do not support viral replication.

4. **Choosing the Right Culture Technique**: Among the options provided, shoot tip culture is particularly effective because it utilizes the apical meristem, which is virus-free. This method allows for the regeneration of a whole plant from this small piece of tissue.

5. **Conclusion**: Therefore, the correct answer to the question is that virus-free plants can be obtained through **shoot tip culture**.

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