In eukaryotes, regulation of gene expression can be done at all the following levels, except

Options

A. Regulation of splicing
B. Formation of primary transcript
C. Transport of hnRNA from the nucleus to the cytoplasm
D. Translational level

Answer

To solve the question regarding the regulation of gene expression in eukaryotes, we need to analyze the options provided and determine which one does not involve regulation at the gene expression level.

### Step-by-Step Solution:

1. **Understanding Gene Expression Regulation**:
Gene expression regulation is a mechanism by which cells control the amount and timing of the appearance of the functional product of a gene. This can occur at various stages, including transcription, post-transcription, and translation.

2. **Evaluating the Options**:
- **Option 1: Regulation of Splicing**:
- Splicing is a post-transcriptional process where introns are removed, and exons are joined together to form mature mRNA. This process allows for the regulation of gene expression by determining which exons are included in the final mRNA. Thus, this option involves regulation.

- **Option 2: Formation of Primary Transcript**:
- The formation of the primary transcript occurs during transcription. This is the first step in gene expression where DNA is transcribed into RNA. Regulation can occur here as it determines how much of the gene product is synthesized. Hence, this option also involves regulation.

- **Option 3: Transport of hnRNA from Nucleus to Cytoplasm**:
- The transport of heteronuclear RNA (hnRNA) from the nucleus to the cytoplasm is a process that occurs after transcription and does not involve the regulation of gene expression itself. This is merely a transport process and does not affect how much gene product is made. Therefore, this option does not involve regulation.

- **Option 4: Transcriptional and Translational Level**:
- This refers to the processes of transcription (synthesis of RNA from DNA) and translation (synthesis of proteins from RNA). Both of these stages are critical points for regulating gene expression. Thus, this option involves regulation.

3. **Conclusion**:
After evaluating all the options, the correct answer is **Option 3: Transport of hnRNA from Nucleus to Cytoplasm**, as this step does not involve the regulation of gene expression.

### Final Answer:
The regulation of gene expression can be done at all the following levels, except **Transport of hnRNA from Nucleus to Cytoplasm**.