Which of the following pairs is incorrect matched ?

Options

A. Genetic code - unambiguous
B. Okazaki fragments - Splicing
C. RNA polymerase - RNA primer
D. Restriction enzymes - Genetic engineering

Answer

To solve the question "Which of the following pairs is incorrectly matched?", we need to analyze each pair provided in the options. The focus will be on understanding the definitions and functions of the terms involved.

### Step-by-Step Solution:

1. **Understanding Genetic Code**:
- The genetic code is described as unambiguous, meaning that each codon (a sequence of three nucleotides) corresponds to a specific amino acid without ambiguity. This means there are no gaps or overlaps in the coding sequence.
- **Hint**: Remember that "unambiguous" refers to the clear and specific relationship between codons and amino acids.

2. **Analyzing Okazaki Fragments**:
- Okazaki fragments are short sequences of DNA that are synthesized discontinuously on the lagging strand during DNA replication. They are formed because DNA polymerase can only synthesize DNA in the 5' to 3' direction, leading to the creation of these fragments.
- The process of splicing, however, refers to the removal of introns from pre-mRNA to produce mature mRNA. This process occurs after transcription and is not related to Okazaki fragments.
- **Hint**: Distinguish between DNA replication (Okazaki fragments) and mRNA processing (splicing).

3. **RNA Polymerase and RNA Primer**:
- RNA polymerase is an enzyme that synthesizes RNA from a DNA template during transcription. It does not act as a primer but is essential for the synthesis of RNA.
- **Hint**: Know the role of RNA polymerase in transcription and how it differs from the role of primers in DNA replication.

4. **Restriction Enzymes**:
- Restriction enzymes are proteins that cut DNA at specific sequences. They are crucial tools in genetic engineering for manipulating DNA.
- **Hint**: Remember that restriction enzymes are used for cutting DNA, which is fundamental in cloning and genetic modification.

5. **Identifying the Incorrect Pair**:
- The incorrect pair is "Okazaki Fragments" and "Splicing". Okazaki fragments are related to DNA replication, while splicing is a process that occurs in mRNA processing.
- Therefore, the answer to the question is that the pair "Okazaki Fragments and Splicing" is incorrectly matched.

### Final Answer:
The incorrectly matched pair is **Okazaki Fragments and Splicing**.