A genetically modified bacteria producing human insulin can be obtained by adding a human insulin gene inside the genetic material of a bacteria. Choose the next step in using the modified bacteria to treat diabetes among the following.

Options

A. Add the altered bacterium to human food.
B. Inject the altered bacterium into the blood of a person with diabetes
C. Put the bacterium into a fermenter to multiply rapidly
D. Use the altered bacterium in a nasal spray.

Answer

To solve the question regarding the next step in using genetically modified bacteria to treat diabetes after incorporating a human insulin gene into their genetic material, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Genetic Modification**:
- The first step involves inserting the human insulin gene into the genetic material of the bacteria. This modification enables the bacteria to produce human insulin.

2. **Evaluating Treatment Options**:
- The question presents several options for the next step after the bacteria have been genetically modified. We need to evaluate each option based on safety and effectiveness.

3. **Analyzing Each Option**:
- **Option A**: Add the altered bacterium to human food.
- This option is not feasible because cooking food would likely kill the bacteria, and there is a risk of immune response and pathogenicity once ingested.

- **Option B**: Inject the altered bacterium into the blood of the person with diabetes.
- This option is also risky as it could provoke an immune response, and there is no guarantee that the bacteria would only produce insulin without causing harm.

- **Option C**: Put the bacterium into a fermenter to multiply rapidly.
- This is a viable option. In a controlled environment, the bacteria can be allowed to multiply and produce insulin, which can then be purified and used for treatment without introducing the bacteria into the patient’s body.

- **Option D**: Use the altered bacterium in a nasal spray.
- Similar to options A and B, this method poses risks of immune response and potential pathogenicity.

4. **Selecting the Best Option**:
- After evaluating all options, **Option C** is the most feasible and safe method for using the genetically modified bacteria to treat diabetes. It allows for the safe production of insulin without introducing the bacteria into the patient's body.

### Conclusion:
The next step in using the modified bacteria to treat diabetes is to **put the bacterium into a fermenter to multiply rapidly** (Option C).

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