Which of the following can be used to insert the recombinant DNA into the animal cell?

Options

A. Agrobacterium
B. Microinjection
C. Biolistic
D. Gene gun

Answer

To solve the question of which method can be used to insert recombinant DNA into an animal cell, we will analyze the options provided: Agrobacterium, Microinjection, Biolostic, and Gene Gun.

### Step-by-Step Solution:

1. **Understanding the Options**:
- **Agrobacterium**: This is a pathogenic bacterium that primarily infects plant cells, particularly dicots. It utilizes a tumor-inducing (TI) plasmid to transfer genes into plants. This method is not suitable for animal cells.

- **Microinjection**: This is a direct gene transfer method where recombinant DNA can be injected directly into the nucleus of an animal cell using a fine glass pipette. This method is specifically designed for animal cells and is effective for inserting DNA directly into the target cells.

- **Biolastic (Gene Gun)**: This method involves shooting microscopic gold or tungsten particles coated with DNA into plant cells. While it can also be used in some animal cells, it is primarily developed for plant transformation. The term "gene gun" refers to the device used in this method.

2. **Identifying the Correct Method**:
- Since Agrobacterium is not applicable to animal cells and the biolastic/gene gun methods are primarily used for plants, the only suitable method for inserting recombinant DNA into animal cells is **Microinjection**.

3. **Conclusion**:
- The correct answer is **Microinjection**.

### Final Answer:
Microinjection is the method that can be used to insert recombinant DNA into an animal cell.