What is the requirement for having sampling ports in a bioreactor?

Options

A. Withdrawing small volumes of culture periodically
B. Introduction of raw material
C. pH control system
D. Temperature control system

Answer

### Step-by-Step Text Solution:

1. **Understanding Sampling Ports**:
- Sampling ports are specific openings in a bioreactor that allow for the extraction of small volumes of the culture without disrupting the overall environment of the bioreactor.

2. **Categories of Sampling Ports**:
- Sampling ports can be categorized based on their functions. The transcript mentions three categories:
- **Category 1**: Bypass sampling ports that allow for the withdrawal of samples.
- **Category 2**: Ports that monitor parameters like pH, dissolved oxygen, and temperature.
- **Category 3**: Ports equipped with sensors for real-time monitoring.

3. **Function of Sampling Ports**:
- The primary function of sampling ports in a bioreactor is to withdraw small volumes of culture periodically for analysis. This helps in monitoring the growth and metabolic activity of the microorganisms or cells within the bioreactor.

4. **Evaluating Options**:
- The question likely presents multiple options regarding the requirements for sampling ports.
- Among the options, the one that states "withdrawing small volume of culture periodically" aligns with the primary function of sampling ports.

5. **Conclusion**:
- Therefore, the requirement for having sampling ports in a bioreactor is to allow for the periodic withdrawal of small volumes of culture for analysis, which is critical for monitoring the bioprocess.