Select the incorrect statement .

Options

A. Selection of recombinations due to inactivation of antibiotic is a cumbersome procedure .
B. Insertional inactivation of `beta ` -galactosidase of leads to colourless colonies .
C. Insertional inactivation of `beta` -galactosidase of leads to blue colourless colonies .
D. Insertional inactivation , the rDNA is inserted within the coding sequence of an enzyme `beta` -galactosidase

Answer

To solve the question of selecting the incorrect statement from the provided options, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Context**: The question asks us to identify an incorrect statement related to recombinant DNA technology, specifically about the selection of recombinant bacteria and the role of beta-galactosidase.

2. **Analyze Each Statement**:
- **Statement A**: "Selection of recombinant due to inactivation of antibiotic is a cumbersome procedure."
- This statement is **correct**. The selection process can be lengthy and complex, as it involves using different antibiotics to identify transformed bacteria.

- **Statement B**: "Insertional inactivation of beta-galactosidase leads to colorless colonies."
- This statement is **correct**. When the gene of interest is inserted into the beta-galactosidase gene, it inactivates the enzyme, resulting in colorless colonies.

- **Statement C**: "Insertional inactivation of beta-galactosidase is related to blue colored colonies."
- This statement is **incorrect**. Blue colonies are produced when beta-galactosidase is active (i.e., when no insertion has occurred). Therefore, this statement contradicts the principle of insertional inactivation.

- **Statement D**: "Insertional inactivation means the recombinant DNA is inserted within the coding sequence of the enzyme beta-galactosidase."
- This statement is **correct**. Insertional inactivation involves inserting the recombinant DNA into the coding sequence of the enzyme, which leads to the inactivation of the enzyme.

3. **Identify the Incorrect Statement**: Based on the analysis, **Statement C** is the incorrect statement.

### Final Answer:
The incorrect statement is: **"Insertional inactivation of beta-galactosidase is related to blue colored colonies."**

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