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Pushpa wants to conduct an experiment to...

Pushpa wants to conduct an experiment to know whether the starch is present in given leaf or not. For this,
a) what apparatus does she require ?
b) what procedure does she follow ?
c) what precautions does she need to take ?

Text Solution

Verified by Experts

Apparatus required: Tripod stand, beaker, test tube, water, spirit lamp/ bunsen burner, petridish, methylated spirit droper, Iodine.

Procedure:
1) Take a leaf of a plant which has soft thin leaves and well exposed to sunlight.
2) Boil the leaf in methylated spirit over a water bath till it becomes pale -white due to the removal of chlorophyll. Opserve the leaf.
3) Spread the leaf in a dish and add a few drops of tincture iodine / betadine solution on it.
4) Observe the leaf.
Observation : The leaf turned into blue -black colour. It indicate the presence of starch.
Inference : It is proved that leaf has starch, that is formed by photosynthesis.
Precautions :
1) Do not boil the methylated spirit test tube directly on flame.
2) Boil the water bath with low flame.
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