Resistance genes present in pBR322 are

Options

A. Penicillin and ampicillin
B. Ampicillin and tetracycline
C. Tetracycline and erythromycin
D. Erythromycin and ampicillin.

Answer

To solve the question regarding the resistance genes present in the plasmid pBR322, we can follow these steps:

### Step-by-Step Solution:

1. **Identify the Vector**:
- Understand that pBR322 is a well-known cloning vector used in molecular biology. It contains specific genes that confer resistance to certain antibiotics.

2. **Recognize the Purpose of Resistance Genes**:
- Resistance genes are crucial for selecting transformed cells. They allow for the identification of cells that have successfully taken up the plasmid containing the gene of interest.

3. **List the Resistance Genes in pBR322**:
- pBR322 contains genes that provide resistance to two specific antibiotics:
- **Ampicillin**
- **Tetracycline**

4. **Evaluate the Options**:
- The question provides several options regarding the resistance genes present in pBR322:
- a) Panicillin and ampicillin
- b) Ampicillin and tetracycline
- c) Tetracycline and erythromycin
- d) Erythromycin and ampicillin

5. **Select the Correct Answer**:
- Based on the information about pBR322, the correct answer is **b) Ampicillin and tetracycline**. These are the two resistance genes present in the plasmid.

### Final Answer:
The resistance genes present in pBR322 are **Ampicillin and Tetracycline**.

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