Statement - 1 : Biolistics method of gene transfer is an example of direct gene transfer.
Statement - 2 : In biolistics method , pBR322 is used.

Options

A. Statement -1 is true , Statement -2 is true, Statement -2 is not the correct explanation of Statement -1.
B. Statement -1 is false , Statement -2 is true.
C. Statement -1 is true , Statement -2 is false.
D. Statement -1 is true , Statement -2 is true, Statement -2 is the correct explanation of Statement - 1.

Answer

To solve the question, we need to analyze both statements provided:

**Statement 1:** Biolistics method of gene transfer is an example of direct gene transfer.

**Statement 2:** In biolistics method, pBR322 is used.

### Step-by-Step Solution:

1. **Understanding Biolistics Method:**
- The biolistics method, also known as the gene gun method, is a technique used to transfer DNA into cells. This method involves the use of high-velocity microparticles (usually made of gold or tungsten) that are coated with DNA. These microparticles are then shot into the target cells, allowing for the direct introduction of the DNA into the cell.

**Hint:** Recall that biolistics is a method that uses physical means to introduce DNA into cells, making it a direct gene transfer method.

2. **Evaluating Statement 1:**
- Since the biolistics method involves directly bombarding cells with DNA-coated particles without the use of a vector, we can conclude that Statement 1 is true. Biolistics is indeed an example of direct gene transfer.

**Hint:** Consider the definition of direct gene transfer and how biolistics fits into that category.

3. **Understanding pBR322:**
- pBR322 is a plasmid vector commonly used in molecular cloning. It is not used in the biolistics method, which does not involve vectors for gene transfer. Instead, the method relies on the physical delivery of DNA into the cells.

**Hint:** Think about the role of vectors in gene transfer methods and whether biolistics requires a vector like pBR322.

4. **Evaluating Statement 2:**
- Since pBR322 is a vector and is not used in the biolistics method, Statement 2 is false.

**Hint:** Reflect on the distinction between methods that use vectors and those that do not.

### Conclusion:
- Statement 1 is true, and Statement 2 is false. Therefore, the correct option is that the first statement is true and the second statement is false.

### Final Answer:
- **Statement 1:** True
- **Statement 2:** False

The correct option is the third one, which states that the first statement is true and the second statement is false.