Which of the following is not present in the stroma of Chloroplast?

Options

A. Double stranded circular DNA
B. 70S ribosome
C. 80s ribosomes
D. Enzymes required for dark reaction

Answer

To determine which of the following is not present in the stroma of chloroplasts, we will analyze each option provided.

### Step-by-Step Solution:

1. **Understanding Stroma**: The stroma is the fluid-filled inner space of chloroplasts that surrounds the thylakoids. It contains various components essential for photosynthesis.

2. **Evaluating Option A: Double-stranded circular DNA**:
- Chloroplasts contain their own DNA, which is double-stranded and circular. This DNA is located in the stroma.
- **Conclusion**: This option is present in the stroma.

3. **Evaluating Option B: 70S ribosome**:
- Chloroplasts contain 70S ribosomes, which are involved in protein synthesis within the organelle.
- **Conclusion**: This option is also present in the stroma.

4. **Evaluating Option C: Enzymes required for dark reactions**:
- The dark reactions (Calvin cycle) occur in the stroma and require specific enzymes for the process of carbon fixation.
- **Conclusion**: This option is present in the stroma.

5. **Identifying the Correct Answer**:
- Since options A, B, and C are all present in the stroma, we need to consider other options not mentioned in the transcript. However, based on the provided information, if there were an option that is not typically found in the stroma, it would be the answer.
- If we assume that the question is asking for something that is not present in the stroma, we can conclude that none of the options A, B, or C are correct as they are all present.

### Final Answer:
Based on the analysis, if the question had options beyond A, B, and C, we would need to identify which of those is not present in the stroma. However, from the options discussed, all are present.