Select the correct statement about the RNAi method.

Options

A. RNAi strategy was taken up to prevent the infestation of nematodes in the roots maize plants.
B. This method involves the use of a complementary dsRNA molecule that binds to ans prevents translation of the mRNA
C. Only RNA genomes of viruses can be used as a source for the complementary RNA
D. Mobile genetic elelments called transposons can replicate only through a DNA intermediate

Answer

To solve the question regarding the RNA interference (RNAi) method, we will analyze the statements provided and determine which one is correct based on our understanding of RNAi.

### Step-by-Step Solution:

1. **Understanding RNA Interference (RNAi)**:
- RNA interference is a biological process where RNA molecules inhibit gene expression or translation by neutralizing targeted mRNA molecules. This is a crucial mechanism for regulating gene expression and defending against viral infections.

2. **Application of RNAi**:
- RNAi can be utilized in biotechnology to create pest-resistant plants. For instance, it can be employed to protect plants from nematode infections, which can significantly reduce crop yields.

3. **Mechanism of RNAi**:
- The process involves the use of complementary double-stranded RNA (dsRNA) that binds to specific mRNA molecules. This binding prevents the translation of the mRNA into proteins, effectively silencing the gene associated with the targeted mRNA.

4. **Evaluating the Statements**:
- **Statement 1**: "RNA interference was taken up to prevent the infestation of nematodes in the roots of maize plants."
- This statement is plausible as RNAi can be used to develop pest-resistant crops, including maize.

- **Statement 2**: "We will use complementary double-stranded RNA which will bind and prevent translation."
- This statement accurately describes the mechanism of RNAi, where complementary dsRNA binds to mRNA to prevent translation.

- **Statement 3**: "Only RNA genomes of viruses can be used as a source of complementary RNA."
- This statement is incorrect. RNAi can utilize various sources of RNA, not just viral genomes. Other sources include mobile genetic elements and transposons.

- **Statement 4**: "These mobile genetic elements, transposome can be replicated via RNA intermediate."
- This statement is also incorrect as it suggests a misunderstanding of the replication process; transposons typically replicate via DNA intermediates.

5. **Conclusion**:
- After analyzing the statements, the correct statement about the RNAi method is **Statement 2**: "We will use complementary double-stranded RNA which will bind and prevent translation."