Which of these enzymes are involved in the DNA isolation of fungi ?

Options

A. Pectinase
B. Cellulase
C. Chitinase
D. Ribonuclease

Answer

To solve the question regarding which enzymes are involved in the DNA isolation of fungi, we will analyze the given options step by step.

### Step-by-Step Solution:

1. **Understanding DNA Isolation**:
- DNA isolation involves breaking down cellular structures to extract DNA. In fungi, this process requires specific enzymes to degrade the cell wall components.

2. **Analyzing the Options**:
- **Option A: Pectinase**: This enzyme primarily breaks down pectin, which is found in plant cell walls. It is not specifically useful for fungi.
- **Option B: Cellulase**: This enzyme breaks down cellulose, which is also not a major component of fungal cell walls.
- **Option C: Chitinase**: This enzyme degrades chitin, a key component of fungal cell walls. It is crucial for breaking down the cell wall to release DNA.
- **Option D: Ribonuclease**: This enzyme degrades RNA, not DNA, and is not involved in the isolation of DNA from fungi.

3. **Identifying the Correct Answer**:
- Among the options, Chitinase (Option C) is the enzyme that specifically aids in breaking down the chitin in fungal cell walls, facilitating the isolation of DNA.

4. **Conclusion**:
- Therefore, the correct answer to the question is **Option C: Chitinase**.