Describe the roles of (a) high temperature, (b) primers, and (c ) bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.

Describe the roles of heat , primers and the bacterium Thermus aquaticus in the process of PCR.

The denaturation process in polymerase chain Reaction (PCR) does not involve

Which type of primer is used the genetic engineering tool -polymerase chain Reaction?

Which of the following statement are correct for the enzyme Taq polymerase? (i) It remains active during the high temperature . (ii) It requies primers for carrying out the process of polymerization. (iii) It induces denaturation of double-stranded DNA.

Which of the following statements are correct for the enzyme Taq polymerase ? (i) It remains active during the hight temperature induced denaturation of dsDNA. (ii) It required primers for carrying out the process of polymerisation. It synthesises the RNA region between the primers, using dNTPs and Mg^(2+) .

During polymerase chain reaction , which primers are used for initiating the process .

In the three step (a,b,c) of polymerase chain reaction, select the correct step

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

In the three steps (a,b,c) of polymerase chain reaction, select the correct step