Denaturation can be achieved at which temperature during PCR?

Options

A. `72^(@)`
B. `94^(@)`
C. `40^(@)C`
D. `25^(@)C`

Answer

**Step-by-Step Solution:**

1. **Understanding PCR:**
- PCR stands for Polymerase Chain Reaction, a technique used to amplify DNA.

2. **Identifying the Denaturation Step:**
- The first step in PCR is denaturation, where the double-stranded DNA is separated into single strands.

3. **Temperature Range for Denaturation:**
- The temperature required for denaturation typically ranges from 94 degrees Celsius to 95 degrees Celsius.

4. **Conclusion:**
- Therefore, denaturation during PCR can be achieved at a temperature of approximately 94 degrees Celsius.

**Final Answer:**
- Denaturation can be achieved at 94 degrees Celsius during PCR.

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