Which of the following should be chosen for best yield if one were to produce a recmobinant protein in large amounts ?

Options

A. A continuous culture system
B. A laboratory flask of large capacity
C. A bioreactor without ports
D. Any of the above

Answer

A

Explanation

`**` After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein one has to consider producing it on a larger scale.
`**` The cells can also be multiplied in a continuous culture system wherein the used medium is drained out from one side while fresh medium is added from the other to maintain the cells in their physiologically most active log/exponential phase. this type of culturing method produced a large biomass leading to higher yields of desired protein.