Home
Class 12
BIOLOGY
Assertion : Unless one cuts the vector a...

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created
Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

A

If both assertion and reason are true and the reason is the correct explanation of the assertion

B

If both assertion and reason are true but reason is not the correct explanation of the assertion

C

If assertion is true but reason is false

D

If both assertion and reason are false

Text Solution

AI Generated Solution

The correct Answer is:
### Step-by-Step Solution: 1. **Understanding the Assertion**: The assertion states that for recombinant DNA to be created, both the vector and the foreign DNA must be cut using the same restriction enzyme. This is because the ends of the DNA fragments need to be compatible for ligation. 2. **Understanding the Reason**: The reason provided explains that when both the vector and the foreign DNA are cut with the same restriction enzyme, they produce DNA fragments with identical sticky ends. These sticky ends are crucial for the ligation process, as they allow the fragments to bond together. 3. **Linking Assertion and Reason**: The assertion is correct because if different restriction enzymes are used, the resulting DNA fragments will have different ends and will not be able to join together. The reason correctly explains why the assertion is true, as it describes the necessity of having compatible sticky ends for successful ligation. 4. **Conclusion**: Both the assertion and the reason are true, and the reason accurately explains the assertion. Therefore, the correct answer is that both the assertion and reason are true, and the reason is the correct explanation of the assertion. ### Final Answer: Both assertion and reason are true, and the reason is the correct explanation of the assertion. ---

### Step-by-Step Solution: 1. **Understanding the Assertion**: The assertion states that for recombinant DNA to be created, both the vector and the foreign DNA must be cut using the same restriction enzyme. This is because the ends of the DNA fragments need to be compatible for ligation. 2. **Understanding the Reason**: The reason provided explains that when both the vector and the foreign DNA are cut with the same restriction enzyme, they produce DNA fragments with identical sticky ends. These sticky ends are crucial for the ligation process, as they allow the fragments to bond together. 3. **Linking Assertion and Reason**: The assertion is correct because if different restriction enzymes are used, the resulting DNA fragments will have different ends and will not be able to join together. The reason correctly explains why the assertion is true, as it describes the necessity of having compatible sticky ends for successful ligation. ...
Promotional Banner

Topper's Solved these Questions

  • BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

    A2Z|Exercise Section C: PREVIOUS YEARS' EXAMINATION QUESTIONS (NEET/AIPMT Question)|15 Videos
  • BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

    A2Z|Exercise Section C: PREVIOUS YEARS' EXAMINATION QUESTIONS (AIIMS Question)|37 Videos
  • BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

    A2Z|Exercise Section A: Topicwise Questions (topic 5: Obtaining The Foreign Gene Product) Downstream Processing)|9 Videos
  • BIOTECHNOLOGY AND ITS APPLICATION

    A2Z|Exercise Section C : PREVIOUS YEARS' EXAMINATIOIN QUESTIONS (Assertion and Reason Type Questions)|4 Videos
  • ECOSYSTEM

    A2Z|Exercise Section-d Chapter-end Test|50 Videos

Similar Questions

Explore conceptually related problems

Assertion: The recombinant DNA molecule cannot be created, unless one cuts the vector & source DNA with same restriction enzyme Reason :When cuts by same enzyme both will have same sticky ends, that can be joined by DNA ligase

A fragment of DNA, cut by a restriction enzyme, forms bonds with other DNA molecules that have

Unless the vector and source DNA are cut, fragments separated and joined, the desired recombinant vector molecule cannot be created. (a) How are the desirable DNA sequence cut ? (b) Explain the technique used to separate the cut fragments. (c) how are the resultant framents joined to the vector DNA molecule ?

DNA fragments cut by restriction enzyme can be separated by

Unless the vector and source DNA are cut, fragments separated and joined, the desired recombinant vector molecule cannot be created. (a) How are the desirable DNA sequences cut ? (b) Explain the technique used to separate the cut fragments. (c) How are the resultant fragments joined to the vector DNA molecule ?

When the genomes of two people are cut using the same restriction enzyme, the length and number of fragments obtained are different, this is called

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form recombinant plasmid using

Restriction enzyme (s) of recombinant DNA technology that make staggered cuts leaving stickly ends is/are

A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section B: Assertion-reasoning Questions)
  1. Assertion : Making curd, bread or wine, which are all microbe mediated...

    Text Solution

    |

  2. Assertion : Asexual reproduction preserves the genetic information, w...

    Text Solution

    |

  3. Assertion : Traditional hybridisation procedures used in plant and ani...

    Text Solution

    |

  4. Assertion : If any piece of DNA is somehow transferred into an alien o...

    Text Solution

    |

  5. Assertion : The first restriction endonuclease was Hine II Reason :Hi...

    Text Solution

    |

  6. Assertion : Besides HindII, today we know more than 900 restriction en...

    Text Solution

    |

  7. Assertion : The convention for naming restriction enzymes is the first...

    Text Solution

    |

  8. Assertion : Nucleases enzymes are of two kinds, exonucleases and endon...

    Text Solution

    |

  9. Assertion : Each restriction endonuclease funtions by 'inspecting' the...

    Text Solution

    |

  10. Assertion: Each restriction endonuclease recognises a specific pallind...

    Text Solution

    |

  11. Assertion : Restriction enzymes cut the strand of DNA a little away fr...

    Text Solution

    |

  12. Assertion : Unless one cuts the vector and the foreign/source DNA with...

    Text Solution

    |

  13. Assertion : In gel electrophoresis, DNA fragments can be separated by ...

    Text Solution

    |

  14. Assertion : If one wants to recover many copies of the target DNA it s...

    Text Solution

    |

  15. Assertion : Selectrable markers helps in identifying and eliminating n...

    Text Solution

    |

  16. Assertion : During transformation, in order to force bacteria to take ...

    Text Solution

    |

  17. Assertion : Nucleic acid is the genetic material of all organisms with...

    Text Solution

    |

  18. Assertion : A large scale production of product involves use of biorea...

    Text Solution

    |

  19. Assertion : Restriction enzyme cuts both vector DNA and foreign DNA at...

    Text Solution

    |