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Assertion : Selectrable markers helps in...

Assertion : Selectrable markers helps in identifying and eliminating non-transformants and selectively permitting the growth of transformants
Reason: If a recombinant DNA bearing gene for resistance to ampicillin is transferred into E.coli cells, the host cells become transformed into ampicillin-resistant cells

A

If both assertion and reason are true and the reason is the correct explanation of the assertion

B

If both assertion and reason are true but reason is not the correct explanation of the assertion

C

If assertion is true but reason is false

D

If both assertion and reason are false

Text Solution

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The correct Answer is:
B
`**` Selectable markers helps in identifying and eliminating non-transformants and selectively permitting the gorwth of transformants
`**` If a recombinant DNA bearing gene for resistance to ampicillin is transferred into E.coli cells, the host cells become transformed into ampicillin-resistant cells
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If a recombinant DNA bearing gene for ampicillin resistance if transferred into E.Coli cells and the host cells are spread on agar plates containing ampicillin, then:

Assertion: A good vector in that whose ori support high copy number Reason :It helps in identifying & eliminating non-transformants & permitting the growth of transformants

Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.

Read the following statement regarding DNA recombinant technology. Identify the statements that are true and false and select the appropriate option. I. Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. II. Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector. III. The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography. IV. A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection. {:(,I,II,III,IV),(1,"False","False","True","True"),(2,"True","True","False","False"),(3,"True","True","True","True"),(4,"False","False","True","False"):}

Recombinant DNA bearing ampicillin resistance gene is passed in E. coli. The latter are spread on agar plates containing ampicillin. Then

(a) Explain how to find whether an E. coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it. (b) What does the ampicillin resistant gene act as in the above case?

A gene whose expression helps to identify transformed cell is known as

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  10. Assertion: Each restriction endonuclease recognises a specific pallind...

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  12. Assertion : Unless one cuts the vector and the foreign/source DNA with...

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  13. Assertion : In gel electrophoresis, DNA fragments can be separated by ...

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  14. Assertion : If one wants to recover many copies of the target DNA it s...

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  15. Assertion : Selectrable markers helps in identifying and eliminating n...

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  16. Assertion : During transformation, in order to force bacteria to take ...

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