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Assertion : Restriction enzyme cuts both...

Assertion : Restriction enzyme cuts both vector DNA and foreign DNA at the same site
Reason: Both plasmid and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA

A

If both assertion and reason are true and the reason is the correct explanation of the assertion

B

If both assertion and reason are true but reason is not the correct explanation of the assertion

C

If assertion is true but reason is false

D

If both assertion and reason are false

Text Solution

Verified by Experts

The correct Answer is:
B
`**` Restriction enzyme cuts both vector DNA and foreign DNA at the same site.
`**` Both plasmids and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA
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Explore conceptually related problems

Plasmids and .............. have the ability to replicate within bacterial cells independent of the control of chromosomal DNA.

Read the following statements and select the correct ones. (i) Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes. (iii) Exonucleases make cuts at specific positions within the DNA. (iii) Hind ll was the first restriction endonuclease to be isolated. (iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA. (v) Presence of more than one recognition sites for a enzyme within the vector complicates the gene cloning.

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : Stickness of the ends facilitates the action of the enzyme DNA polymerase

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : In this process, there is net gain of twenty four molcules of ATP.

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA polymerase

Assertion : Restriction enzymes cut the strand of DNA a little away from the centre of the palindromic sites Reason: Restriction enzymes cut the strads of DNA between the same two bases on the opposite strand.

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

Assertion: The cut pieces of DNA are linked with plasmid DNA. Reason: Plasmid DNA fails to act as vectors.

Which one is correct answer for plasmid (i) It can be readily isolated from cells (ii) It possesses a single recognition site for one restriction enzyme (iii) It should have high molecular weight (iv) Plasmids and bacteriophages have the ability to replicate within bacteria

A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section B: Assertion-reasoning Questions)
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