In a mixture,DNA fragments are separated by:

Options

A. Bioprocess engineering
B. Restriction digestion
C. Electrophoresis
D. Polymerase Chain Reaction

Answer

To solve the question of how DNA fragments are separated in a mixture, we can follow these steps:

### Step 1: Understand the Options
We have four options to consider:
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction (PCR)

### Step 2: Analyze Each Option
- **Bioprocess Engineering**: This is a broader field that deals with the design and development of equipment and processes for the manufacturing of products like pharmaceuticals and biofuels. It does not specifically refer to the separation of DNA fragments.

- **Restriction Digestion**: This process involves cutting DNA into smaller fragments using restriction enzymes. While it is a method to manipulate DNA, it does not separate DNA fragments in a mixture.

- **Electrophoresis**: This is a laboratory technique used to separate charged molecules, such as DNA fragments, based on their size and charge. It involves applying an electric current to a gel matrix, allowing smaller fragments to move faster than larger ones.

- **Polymerase Chain Reaction (PCR)**: PCR is a technique used to amplify specific DNA sequences. It does not separate DNA fragments but rather makes many copies of a specific DNA segment.

### Step 3: Identify the Correct Answer
Given the analysis, the correct answer to the question of how DNA fragments are separated in a mixture is **Electrophoresis**. This technique allows for the separation of DNA fragments based on size and charge, making it the most suitable method for this purpose.

### Final Answer:
**Electrophoresis** is the method used to separate DNA fragments in a mixture.

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