Spooling is:-

Options

A. Amplification of DNA
B. Cutting of seperated DNA bands from the agarose gel
C. Transfer of separated DNA framents to synthetic membranes
D. Collection of isolated DNA

Answer

**Step-by-Step Solution:**

1. **Understanding Spooling**: Spooling refers to a specific method used in molecular biology for the extraction of DNA. It involves collecting DNA that has been precipitated out of a solution.

2. **DNA Extraction Process**: Initially, DNA is extracted from cells, resulting in a solution where DNA is present in a cloudy form. This is typically done through various techniques that break down cell membranes and release DNA into a solution.

3. **The Spooling Process**: After the DNA has been extracted, it is often in a solution that contains various impurities. To isolate the DNA, a glass rod is used to physically collect the DNA strands. This is done by gently twirling the rod in the solution, which causes the DNA to adhere to it.

4. **Identifying the Correct Option**: In the context of the question, we need to identify what spooling specifically refers to. The options provided include:
- Amplification of DNA
- Cutting of separated DNA bonds from agarose gel
- Transfer of separated DNA fragments to synthetic membrane
- Collection of isolated DNA

Among these options, spooling is best described as the "collection of isolated DNA" after it has been extracted and precipitated.

5. **Conclusion**: Therefore, the correct answer to the question "Spooling is:" is the collection of isolated DNA.

**Final Answer**: Spooling is the collection of isolated DNA.

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