During in vitro synthesis of DNA, a researcher used 2'-3'—dideoxycytidine triphosphate as raw nucleotide in place of 2'-deoxy cytidine triphosphate other conditions remaining as standard. Will further polymerisation of DNA continue upto the end or not? Explain.

Explanation

**Step-by-Step Solution:**

1. **Understanding the Components**: In DNA synthesis, nucleotides are the building blocks. Each nucleotide consists of a sugar (deoxyribose), a phosphate group, and a nitrogenous base. In this case, the researcher is using 2'-3'-dideoxycytidine triphosphate (ddCTP) instead of the normal 2'-deoxycytidine triphosphate (dCTP).

2. **Difference Between dCTP and ddCTP**: The key difference between dCTP and ddCTP is that ddCTP lacks the hydroxyl (OH) group at the 2' and 3' positions of the sugar. This absence of the OH group is crucial for the polymerization process.

3. **Role of the 3' OH Group**: During DNA synthesis, DNA polymerase adds nucleotides to the growing DNA strand. This addition occurs through a reaction where the 3' OH group of the last nucleotide in the chain attacks the phosphate group of the incoming nucleotide. This reaction releases a molecule of water and forms a phosphodiester bond.

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