a. What are molecular scissors ? Give one example .
b. Explain their role in recombinant DNA technology .

Explanation

a. These are restriction endonucleases which cut the DNA molecule into fragments with sticky ends.
b. The enzyme restriction endonuclease cleaves DNA at specific site resulting in the formation of sticky ends. In practice, the digestion by the restriction enzyme is performed by incubation of the puri-fied DNA molecules with the specific enzyme keeping all other conditions at the optimum level and checked by using agarose gel electrophoresis .