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Explain the importance of (a) ori, (b) a...

Explain the importance of (a) ori, (b) `amp^(R)` and (c) rop in the E. coli vector shown below .

Text Solution

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a = ori = origin or replication
b = `amp^(R)` = ampicillin resistance genes
c = rop = proteins involved in replication of plasmid .
ori = origin of replication is a specific protion of plasmid genome that serves as start signal for self replication .
Plasmids are characterised to possess some important genes like the antibiotic resistance gene like `amo^(R) , tet^(R)` .
The proteins produced by rop genes will help in the replication of plasmid .
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