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If a certain plant when introduced into ...

If a certain plant when introduced into new environment neither produces seeds nor it responds to vegetative reproduction (Propagation ), how can more plants be produced of its kinds from this plant ? State the method .

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At this stage, only tissue culture is the best method to produce more plants of its own kind . For this purpose following steps are to be taken.
i. A tissue from parental is to be sterilised.
ii. A suitable culture medium is provided to the separated tissue.
iii. Invoculation of this tissue and maintenance in aseptic conditions.
iv . Certain growth promoting hormones are also used for stimulating growth and differentiation .
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